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Text File | 1995-12-17 | 2.7 KB | 99 lines | [TEXT/R*ch] |
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- CLUSTAL W(1.4) Multiple Sequence Alignments
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- DATA (sequences)
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- /INFILE=file.ext :input sequences.
- /PROFILE1=file.ext and /PROFILE2=file.ext :profiles (old alignment).
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- VERBS (do things)
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- /OPTIONS :list the command line parameters
- /HELP or /CHECK :outline the command line params.
- /ALIGN :do full multiple alignment
- /TREE :calculate NJ tree.
- /BOOTSTRAP(=n) :bootstrap a NJ tree (n= number of bootstraps; def. = 1000).
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- PARAMETERS (set things)
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- ***General settings:****
- /INTERACTIVE :read command line, then enter normal interactive menus
- /QUICKTREE :use FAST algorithm for the alignment guide tree
- /NEWTREE= :file for new guide tree
- /USETREE= :file for old guide tree
- /NEGATIVE :protein alignment with negative values in matrix
- /OUTFILE= :sequence alignment file name
- /OUTPUT= :GCG, GDE, PHYLIP or PIR
- /OUTORDER= :INPUT or ALIGNED
- /CASE :LOWER or UPPER (for GDE output only)
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- ***Fast Pairwise Alignments:***
- /KTUP=n :word size /TOPDIAGS=n :number of best diags.
- /WINDOW=n :window around best diags. /PAIRGAP=n :gap penalty
- /SCORE :PERCENT or ABSOLUTE
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- ***Slow Pairwise Alignments:***
- /PWMATRIX= :BLOSUM, PAM, ID or filename
- /PWGAPOPEN=f :gap opening penalty /PWGAPEXT=f :gap opening penalty
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- ***Multiple Alignments:***
- /MATRIX= :BLOSUM, PAM, ID or filename
- /GAPOPEN=f :gap opening penalty /GAPEXT=f :gap extension penalty
- /ENDGAPS :no end gap separation pen. /GAPDIST=n :gap separation pen. range
- /NORGAP :Residue specific gaps off /NOHGAP :hydrophilic gaps off
- /HGAPRESIDUES= :list hydrophilic res. /MAXDIV=n :% ident. for delay
- /TYPE= :PROTEIN or DNA /TRANSITIONS :transitions NOT weighted.
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- ***Trees:*** /SEED=n :seed number for bootstraps.
- /KIMURA :use Kimura's correction. /TOSSGAPS :ignore positions with gaps.
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- clustalw option list:-
- /help
- /check
- /options
- /infile=filename
- /profile1=filename
- /profile2=filename
- /align
- /newtree=filename
- /usetree=filename
- /bootstrap
- /tree
- /quicktree
- /interactive
- /type=protein OR dna
- /matrix=filename
- /negative
- /gapopen=f
- /gapext=f
- /endgaps
- /norgap
- /nohgap
- /hgapresidues=string
- /maxdiv=n
- /gapdist=n
- /pwmatrix=filename
- /pwgapopen=f
- /pwgapext=f
- /ktuple=n
- /window=n
- /pairgap=n
- /topdiags=n
- /score=percent OR absolute
- /transitions
- /seed=n
- /kimura
- /tossgaps
- /debug=n
- /output=gcg OR gde OR pir OR phylip
- /outputtree=nj OR phylip OR dist
- /outfile=filename
- /outorder=input OR aligned
- /case=lower OR upper
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